A Study Protocol for Pharmaceutico-Analytical study of Rajat Sindoor and evaluation of its Anti-Cancer Activity against Leukaemia using HL60 and K562 cell line model

Abstract

Introduction: Leukaemia is a disease of haematological malignancies that result from the abnormal multiplication of developing leukocytes. A patient suffering through leukaemia experiences an aberrant blood cell synthesis, usually involving leukocytes (white blood cells). Leukaemia is categorized into two groups, based on the cell types that are mostly involved: Lymphoid and Myeloid, also based on the disease's natural course: Acute and Chronic. Neoplasms and Vyadhi Arbuda, which is mentioned in the classics of Ayurveda (Charaka Samhita and Sushruta Samhita), are comparable. Rasa Aushadhis have many therapeutic advantages in many chronic health aspects such as CKD, Cardiac conditions, Inflammatory conditions, bleeding disorders, cancers, etc. Rajat Sindoor is one of the effective Rasa Kalpas found no previous work on in-vitro investigation to assess the anti-cancer activity w. s. r. to Leukaemia.

Materials and Methods: Rajat Sindoor will be prepared as per classical reference. The prepared formulation will be assessed with organoleptic characters and physio-chemical parameters. The Experimental study will be carried out according to the OECD guidelines.

Result: The analytical parameters will be assessed to establish pharmaceutical standardization. The experimental study and statistical analysis will be done accordingly.

Discussion: The purpose of the current study is to evaluate Anti-cancer activity of Rajat Sindoor w. s. r. Leukaemia using HL60 and K562 cell line model. A small step to fill the research gap in the treatment aspect of Leukaemia from Ayurvedic point of view will be done. If significant positive results are obtained in this work, then it will be a valuable contribution in the treatment of leukaemia.