Comprehensive analysis of the Anti-Inflammatory Activity of Champak Agad: An In-Vitro Study
Abstract
Introduction: Prolonged inflammation can cause several health problems like cardiovascular diseases, arthritis, Crohn's disease, ulcerative colitis, and even cancer. Herbal anti-inflammatory formulations can be used to reduce the symptoms of inflammation as they have fewer side effects compared to synthetic drugs. One such herbal formulation is Champak Agad. This study was implemented to evaluate the possibility of Champak Agad's anti-inflammatory properties, which may be important for treating inflammatory diseases.
Materials and Methods: Champak Agad was prepared according to the classical reference. The anti-inflammatory activity of Champak Agad was examined using an egg albumin protein denaturation assay in which the aqueous and alcoholic extract of the drug was used and diclofenac was used a standard drug.
Result: In this study, the drug extracts showed varying degrees of inhibition according to different drug concentrations. This investigation demonstrated that the aqueous extract exhibited higher anti-inflammatory activity than the alcoholic extract. The aqueous extract showed an inhibition rate of 85% with a 200mg/ml drug dose and the inhibition rate observed with the alcoholic extract was 82% with a 200mg/ml drug dose.
Discussion: By inhibiting protein denaturation in a concentration-dependent manner, the current study shows that Champak Agad extracts have strong anti-inflammatory properties.
Downloads
References
Tripathi B. Ashtang Haridyam Uttarsthan. Delhi: Chaukhambha Sanskrit Pratishthna; 2012. p. 37/71.
Gambhire M, Juvekar A, Wankhede S. Evaluation of anti-inflammatory activity of methanol extract of Barleria Cristata leaves by in vivo and in vitro methods. Internet J Pharmacol. 2008;7(1).
Dharmadeva et al. Anti-inflammatory activity of Ficus racemosa bark – An in vitro study. Int Q J Res Ayurveda. 2019.
Aggarwal BB, Gupta SC, Sung B. Curcumin: an orally bioavailable blocker of TNF and other pro-inflammatory biomarkers. Br J Pharmacol. 2013 Aug;169(8):1672–92. doi:10.1111/bph.12131. PMID: 23425071; PMCID: PMC3753829.
Sadeghi M, Dehnavi S, Asadirad A, et al. Curcumin and chemokines: mechanism of action and therapeutic potential in inflammatory diseases. Inflammopharmacol. 2023;31:1069–93.
Kuo CL, Chi CW, Liu TY. The anti-inflammatory potential of berberine in vitro and in vivo. Cancer Lett. 2004;203(2):127–37.
Zeng W, Fang Y, Mo S, Shen C, Yang H, Luo G, Xiao L, Zhan R, Yan P. The underlying mechanisms exploration of Rubia cordifolia L. extract against rheumatoid arthritis by integrating network pharmacology and metabolomics. Drug Des Devel Ther. 2023;439–57.
Subhan F, Karim N, Ibrar M. Anti-inflammatory activity of methanolic and aqueous extracts of Valeriana wallichii DC rhizome. Pak J Plant Sci. 2007;13:103–8.
Sundaram JK, Hanumanthappa M, Kandagalla S, Shekarappa SB, Gollapalli P, Hani U. Evaluating the anti-inflammatory potential of Mesua ferrea Linn. stem bark through network pharmacology approach. J Appl Biol. [Year, Volume(Issue): Page numbers].
Wu SQ, Otero M, Unger FM, Goldring MB, Phrutivorapongkul A, Chiari C, Kolb A, Viernstein H, Toegel S. Anti-inflammatory activity of an ethanolic Caesalpinia sappan extract in human chondrocytes and macrophages. J Ethnopharmacol. 2011 Nov 18;138(2):364–72.
Healthline. Ways to improve digestion. Available from: https://www.healthline.com/nutrition/ways-to-improve-digestion.
Pålsson-McDermott EM, O’Neill LAJ. Targeting immunometabolism as an anti-inflammatory strategy. Cell Res. 2020;30:300–14.
